Arizona Andrology Laboratory & Cryobank
Providing state-of-the-art male fertility testing, processing, cryopreservation, and storage

Gateway Wellness Center - 850 N. Kolb Road, Tucson, AZ 85710

Phone: 520-885-2689 - Fax: 520-731-0715


Anti-Sperm Antibodies
The presence of anti-sperm antibodies is associated with immunological fertility impairment. Semen specimens with anti-sperm antibodies (ASA) can often present themselves with motile sperm caught in clumps together (agglutinated). While there are several classes of ASA, only those of the IgG and IgA classes are clinically significant. IgA antibodies rarely if ever occur without IgG antibodies also being present. Therefore, testing for IgG is sufficient for routine screening of semen samples for anti-sperm antibodies. The ASA assay uses the direct method of testing by mixing fresh, untreated semen with latex particles that have been coated with human IgG. To this mixture is added a monospecific antihuman IgG antiserum. The formation of agglutinates between the particles and motile sperm indicates the presence of IgG antibodies on the sperm. A Complete Semen Analysis should be ordered along with the assay. 

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